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Literature summary extracted from

  • Spedaletti, V.; Polticelli, F.; Capodaglio, V.; Schinina, M.E.; Stano, P.; Federico, R.; Tavladoraki, P.
    Characterization of a lysine-specific histone demethylase from Arabidopsis thaliana (2008), Biochemistry, 47, 4936-4947 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.67 glycerol 50% increase of activity in the presence of 30% (v/v) glycerol as compared to that in the presence of 0-10% (v/v) glycerol Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.67 expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.67 K+ strong inhibition at 50 mM Arabidopsis thaliana
1.14.11.67 Mg2+ 75% inhibition at 50 mM Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.67 0.0089
-
 [histone H3]-N6-methyl-L-lysine4 at pH 8.0 and 25°C Arabidopsis thaliana
1.14.11.67 0.0107
-
 [histone H3]-N6,N6-dimethyl-L-lysine4 at pH 8.0 and 25°C Arabidopsis thaliana
1.14.11.67 0.0128
-
 [histone H3]-N6,N6-dimethyl-L-lysine4 in 10% (v/v) glycerol, at pH 8.0 and 25°C Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.67 [histone H3]-N6,N6-dimethyl-L-lysine4 + 2-oxoglutarate + O2 Arabidopsis thaliana
-
 [histone H3]-N6-methyl-L-lysine4 + succinate + formaldehyde + CO2
-
 ?
1.14.11.67 [histone H3]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2 Arabidopsis thaliana
-
 [histone H3]-L-lysine4 + succinate + formaldehyde + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.67 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.67 Ni2+-charged resin column chromatography and HiTrap heparin column chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.67 additional information no activity with [histone H3]-N6,N6-dimethyl-L-lysine9, [histone H3]-N6,N6-dimethyl-L-lysine27, spermine, spermidine, and putrescine Arabidopsis thaliana ?
-
-
1.14.11.67 [histone H3]-N6,N6-dimethyl-L-lysine4 + 2-oxoglutarate + O2
-
 Arabidopsis thaliana [histone H3]-N6-methyl-L-lysine4 + succinate + formaldehyde + CO2
-
 ?
1.14.11.67 [histone H3]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2
-
 Arabidopsis thaliana [histone H3]-L-lysine4 + succinate + formaldehyde + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.67 ? x * 94000, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.14.11.67 histone H3 lysine 4 demethylase
-
 Arabidopsis thaliana
1.14.11.67 LSD1
-
 Arabidopsis thaliana
1.14.11.67 lysine-specific demethylase 1
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.11.67 0.0057
-
 [histone H3]-N6,N6-dimethyl-L-lysine4 in 10% (v/v) glycerol, at pH 8.0 and 25°C Arabidopsis thaliana
1.14.11.67 0.0083
-
 [histone H3]-N6-methyl-L-lysine4 at pH 8.0 and 25°C Arabidopsis thaliana
1.14.11.67 0.0113
-
 [histone H3]-N6,N6-dimethyl-L-lysine4 at pH 8.0 and 25°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.67 8
-
-
 Arabidopsis thaliana

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.14.11.67 50
-
 at pH 8.0 and 25°C Arabidopsis thaliana K+